Reduction in viability oh human cervix carcinoma HeLa cells by p21 gene transferred with block polymeric dimethylaminoethyl methacrylate-based carrier

Background. Various cationic polymers have been proposed for use as carriers of genetic materials in biotechnology and biomedicine. The poly(2-dimethylamino)ethyl-methacrylate (poly(DMAEMA)) demonstrated high transfection efficiency low cytotoxicity when used a gene delivery system. aim the present study was to evaluate ability poly(DMAEMA)-block-poly(N-vinylpyrrolidone)-co-(butyl-acrylate)-co-2-aminoethyl methacrylate carrier, BP83-1, deliver p21 into human cervix carcinoma HeLa cells define its effects on viability tumor vitro. Methods. Transfection assay, Western-blot analysis, MTT test, DNA comet analysis alkaline conditions, diphenylamine assay fragmentation (Barton’s assay), FACS cell cycling. Results. BP83-1 polymer effectively transferred pFlag-P21WT plasmid containing cells. level BP83-1-facili­tated significantly higher than achieved with linear polyethyleneimine (PEI). A significant (26.1 % 40.1 %) reduction transfected pDNA/BP83-1 pDNA/PEI polyplexes detected compared non-transfected reverse dependence between elevated amount reduced Cdk2 observed number G1 phase cycle increased from 54.9 65.8 64.9 after their pFlag-P21WT/BP83-1 pFlag-P21WT/PEI polyplexes, correspondingly. Besides, an single-strand breaks content fragmented polyplexes. dama­ging carrier polyplex were less pronounced treated cells, such PEI polyplex. Conclusion. An effective transfer demonstrated. overexpression led inhibition damage, blocking progression S via cyclin-dependent kinase 2 (Cdk2) accumulation phase.